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KMID : 0381120220440010039
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Genes and Genomics
2022 Volume.44 No. 1 p.39 ~ p.51
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Physiological characteristics and miRNA sequencing of two root zones with contrasting ammonium assimilation patterns in Populus
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Zhou Jing
Wu Jiang Ting
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Abstract
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Background: The net ammonium fluxes differ among the different root zones of Populus, but the physiological and microRNA regulatory mechanisms are unclear.
Objective: To elucidate the physiological and miRNA regulatory mechanisms, we investigated the two root zones displaying significant differences in net NH4+ effluxes of P.?×?canescens.
Methods: Populus plantlets were cultivated with 500 μM NH4Cl for 10 days. Six plants were randomly selected to determine the net NH4+ fluxes using a noninvasive microtest technique. High-throughput sequencing were used to determine the dynamic expression profile of miRNA among the different root zones of Populus.
Results: Net NH4+ efflux in zone I (from 0 to 40 mm from the root apex) was ? 19.64 pmol cm?2 s?1 and in zone II (from 40 to 80 mm) it was ? 43.96 pmol cm?2 s?1. The expression of eleven miRNAs was significantly upregulated, whereas fifteen miRNAs were downregulated. Moreover, eighty-eight target genes of the significantly differentially expressed miRNAs were identified in root zone II compared with zone I. Particularly, ptc-miR171a/b/e and their target, SCL6, were found to be important for the difference in net NH4+ effluxes in the two root zones. Moreover, the expression of the target of ptc-miR169d, NFYA3 was upregulated in root zone II compared with root zone I, contributing to increased NH4+ efflux and decreased NH4+ assimilation in root zone II.
Conclusion: These results indicate that miRNAs regulate the expression levels of their target genes and thus play key roles in net NH4+ fluxes and NH4+ assimilation in different poplar root zones.
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KEYWORD
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Net NH4 + fluxes, MicroRNAs, Poplar, Root zones, Ammonium
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